A new digital transmitted light inverted microscope for routine cell culture from Leica Microsystems enables laboratory personnel to easily check and document cell growth. By consistently measuring confluency with the Mateo TL system, users can be confident in the readiness of samples for downstream experiments.

“During the sample preparation process, subjective judgments are often used for sample readiness assessments, which can compromise the quality and the outcome of experiments," explained Jennifer Kulhei, product manager, life science and research microscopy at Leica Microsystems. “Mateo TL helps to address this with more consistent confluency evaluations, no matter who is doing the check or what type ofSource: Leica Microsystems experiment is being undertaken.”

With Mateo TL, laboratories can establish a reliable criterion for evaluating confluency, improving experiment reproducibility. The confluency algorithm measures the percentage of cell coverage in the image, enabling consistent measurements across multiple users. Additionally, users can work in a sterile environment to minimize contamination risk by placing Mateo TL directly under the flow hood, ensuring the integrity of their experimental outcomes.

The user-friendly microscope is easy to set up and use and is designed to support cell culture checks for a range of users, from principal investigators and postdoctoral researchers to student researchers and lab technicians, all of whom may have varying levels of microscopy expertise. The microscope can be set up in less than a minute, providing a ready-to-use system that team members can start working on immediately. Once set up, cell culture checks are streamlined with Mateo TL's user-friendly software interface, enabling simple live viewing and image acquisition.