Environmental, Health and Safety

Watch: Engineers Grow Functioning Human Muscle in a Lab from Just Human Skin Cells

09 January 2018

A cross section of a muscle fiber grown from induced pluripotent stem cells. The green indicates muscle cells, the blue is cell nuclei, and the red is the surrounding support matrix for the cells. Source: Duke UniversityA cross section of a muscle fiber grown from induced pluripotent stem cells. The green indicates muscle cells, the blue is cell nuclei, and the red is the surrounding support matrix for the cells. Source: Duke University

Biomedical engineers have grown the first functioning human skeletal muscle from induced pluripotent stem cells.

This development builds on work published in 2015 when researchers at Duke University grew the first functioning human muscle tissue from cells that were obtained from muscle biopsies. The ability to start from cellular scratch using non-muscle tissue will allow scientists to grow way more muscle cells, provide an easier path to genome editing and cellular therapies, and develop individually tailored models of rare muscle diseases for drug discovery and basic biology studies.

"Starting with pluripotent stem cells that are not muscle cells, but can become all existing cells in our body, allows us to grow an unlimited number of myogenic progenitor cells," said Nenad Bursac, professor of biomedical engineering at Duke University. "These progenitor cells resemble adult muscle stem cells called 'satellite cells' that can theoretically grow an entire muscle starting from a single cell."

In the previous work, Bursac and team started with small samples of human cells obtained from muscle biopsies, called “myoblasts,” that had already progressed beyond the stem cell stage, but hadn’t become mature muscle fibers. They grew myoblasts by many folds, and then put them into supportive 3D scaffolding filled with a nourishing gel that allowed them to form aligned and functioning human muscle fibers.

In the new study, the researchers started with human-induced pluripotent stem cells. These cells are taken from adult, non-muscle tissues, like skin or blood, and reprogrammed to revert to a primordial state. The pluripotent stem cells are grown while being flooded with a molecule called Pax7. Pax7 signals the cells to start becoming muscle.

As the cells proliferated, they became very similar to adult muscle stem cells, but not quite as robust. While previous studies had accomplished this feat, nobody has been able to then grow these intermediate cells into functioning skeletal muscle.

The Duke researchers have succeeded where previous attempts failed.

"It's taken years of trial and error, making educated guesses and taking baby steps to finally produce functioning human muscle from pluripotent stem cells," said Lingjun Rao, a postdoctoral researcher in Bursac's laboratory and first author of the study. "What made the difference are our unique cell culture conditions and 3-D matrix, which allowed cells to grow and develop much faster and longer than the 2-D culture approaches that are more typically used."

When the cells were on their way to becoming muscle, Bursac and Rao stopped providing the Pax7 signaling molecule and started giving the cells the support and nourishment they needed to fully mature.

During the research, the researchers show that after two to four weeks of 3D culture, the resulting muscle cells form muscle fibers that contract and react to external stimuli, like electrical pulses and biochemical signals mimicking neuronal inputs, just like native muscle tissue.

They also implanted the newly grown muscle fibers into adult mice and showed that they survive and function for at least three weeks, while progressively integrating into the native tissue through vascularization.

The resulting muscle is not as strong as native muscle tissue, and falls short of the muscle grown in the previous study that started from muscle biopsies. Despite this, the researchers say that this muscle still holds potential that the stronger, older relative doesn’t.

The pluripotent stem cell-derived muscle fibers develop reservoirs of “satellite-like cells” that are necessary for normal adult muscles to repair damage, while the muscle from the previous study had much fewer of these cells. The stem cell method is capable of growing many more cells from a smaller starting batch than the biopsy method.

Both of the advantages point toward a possibility of using this new method for regenerative therapies and for creating models of rare diseases for future studies and individualized healthcare.

"The prospect of studying rare diseases is especially exciting for us," said Bursac. "When a child's muscles are already withering away from something like Duchenne muscular dystrophy, it would not be ethical to take muscle samples from them and do further damage. But with this technique, we can just take a small sample of non-muscle tissue, like skin or blood, revert the obtained cells to a pluripotent state, and eventually grow an endless amount of functioning muscle fibers to test."

The technique also holds promise for being combined with genetic therapies. Researchers could fix genetic malfunctions in the induced pluripotent stem cells derived from a patient and then grow small patches of completely healthy muscle. While this couldn’t heal or replace an entire body’s worth of diseased muscle, it could be used in tandem with more widely targeted genetic therapies or to heal more localized problems.

The researchers are currently refining the technique to grow more robust muscles and beginning work to develop new models of rare muscle diseases.

The paper on this research was published in Nature Communications.

To contact the author of this article, email Siobhan.Treacy@ieeeglobalspec.com

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